Tissue Trauma Scoring criteria
| Trauma Score | Cellular architecture |
| Grade 0 (no trauma to the serosa) | Nuclei of serosal and bounding muscularis externa cells are smooth, oval and uniformly shaped. |
| Grade 1 (minor trauma to the serosa) | Elongation and mild hyperchromasia of nuclei in muscle/connective tissue in both the serosa and muscularis propria externa outer longitudinal layer, but in less than 50% of cells in our region of interest (ROI), representing trauma to the cells. |
| Grade 2 (significant trauma to the serosa) | Clear and significant damage to the serosa and muscularis propria externa outer longitudinal layer, with more than 50% of nuclei in the cells in our ROI appearing significantly elongated and thinned and there is the presence of multiple hyperchromatic nuclei. |
| Grade 3 (complete denudation of the serosa) | The serosa and muscularis externa longitudinal layers are both compressed, with evidence of denudation of the serosa and trauma extending to the muscularis propria inner circular layer. |